huprottm human proteome microarrays v4.0  (CDI Laboratories)

Journal: Communications Medicine

Article Title: Preclinical characterization of an active immunotherapy targeting calcitonin gene-related peptide

doi: 10.1038/s43856-025-00870-2

Figure Lengend Snippet: Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome Microarray screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.

Article Snippet: Guinea pig sera collected at week 15 post p4796kb immunization were assessed for their potential off target binding using HuProtTM Human Proteome Microarrays v4.0 (Cambridge Protein Arrays Ltd., Cambridge, UK) with over 20,000 individual proteins, representing more than 16,000 human genes, including the peptides of the calcitonin/CGRP peptide family.

Techniques: Binding Assay, Affinity Purification, Microarray, Blocking Assay, Synthesized, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Applications of Protein Microarrays in Biomarker Discovery for Autoimmune Diseases

doi: 10.3389/fimmu.2021.645632

Figure Lengend Snippet: Major protein microarray platforms for biomarker discovery in autoimmune diseases.

Article Snippet: The HuProtTM Human Proteome Microarray V1.0 was designed by Zhu lab at Johns Hopkins School of Medicine and produced by CDI Laboratories around 2012, containing 16,368 recombinant GST-His-tagged human proteins expressed in S. cerevisiae , and the current v4.0 contains more than 21,000 unique proteins.

Techniques: Microarray, Biomarker Discovery, Protein Array, Recombinant, In Silico, Selection, In Situ, Peptide Microarray, Ab Array, Bioprocessing

Journal: Frontiers in Immunology

Article Title: Applications of Protein Microarrays in Biomarker Discovery for Autoimmune Diseases

doi: 10.3389/fimmu.2021.645632

Figure Lengend Snippet: Selective application of protein microarrays in biomarker discovery for autoimmune diseases.

Article Snippet: The HuProtTM Human Proteome Microarray V1.0 was designed by Zhu lab at Johns Hopkins School of Medicine and produced by CDI Laboratories around 2012, containing 16,368 recombinant GST-His-tagged human proteins expressed in S. cerevisiae , and the current v4.0 contains more than 21,000 unique proteins.

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Western Blot, Microarray, Immunopeptidomics, Protein Array, Diagnostic Assay, Recombinant, Purification, Derivative Assay, Competitive ELISA, Peptide Microarray, Software, Construct, Bioprocessing, Ab Array, Clinical Proteomics, Expressing, Amplified Luminescent Proximity Homogenous Assay, Multiplex Assay, Glycoproteomics, Membrane, Sequencing, Immunofluorescence, Staining, Virus, Immunohistochemistry, cDNA Library Assay, Clone Assay, Suspension, Apoptosis Assay, Stripping Membranes, In Silico, Activity Assay, Cytokine Assay